Figure 4. Maternal postpartum Tregs mitigate IFN-γ responsiveness and mediate resiliency to fetal resorption in secondary pregnancy.

a, Representative plots illustrating the majority (>96%) of Foxp3⁺ cells are derived from adoptively transferred CD4 in DT treated Foxp3^DTR/DTR mice. b, IFN-γ producing 2W1S⁺ cells among CD45.1⁺CD45.2⁻ cells, accumulation of 2W1S⁺Foxp3⁺ cells, and Helios expression among 2W1S⁺Foxp3⁺ Tregs five days after Lm-2W1S inoculation. c, Percent fetal resorption during primary (open) or secondary (shaded) allogeneic pregnancy for Foxp3^WT/WT, Foxp3^DTR/WT, or Foxp3^DTR/DTR females five days after DT initiation beginning midgestation. Bar, mean ± one standard error.