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. Author manuscript; available in PMC: 2013 Apr 4.
Published in final edited form as: Nature. 2012 Aug 19;490(7418):107–111. doi: 10.1038/nature11351

Figure 2. Resident peritoneal macrophages are critical for the early FlaTox response in vivo.

Figure 2

(a–f) Mice were injected intraperitoneally with FlaTox and rectal temperature (a,c,e) or hematocrit (b,d,f) were measured after 30 minutes or at indicated times. (a–b) Bone marrow chimeric mice (KO = Nlrc4−/−; n=5). (c–d) Macrophage-depleted wild-type (B6) mice (n=3–6). (e–f) CD11b+ cell depleted FVB:CD11b-DTR mice (n=6–7). (g) Nlrc4−/− host mice injected intraperitoneally with 107 resident (Res) or thioglycollate-elicited (Thio) peritoneal cells or BMDM of indicated genotype. Rectal temperature was measured 30 minutes after intraperitoneal FlaTox (8 μg/g PA + 4 μg/g LFn-FlaA) injection. Data shown (± s.e.m.) are pooled from multiple experiments (g) or representative of at least three (a–f) independent experiments. ** p < 0.01; *** p = 0.0007 (Mann-Whitney t-test).