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. Author manuscript; available in PMC: 2013 Nov 1.
Published in final edited form as: Traffic. 2012 Sep 13;13(11):1466–1480. doi: 10.1111/j.1600-0854.2012.01407.x

Figure 1.

Figure 1

BODIPY-chol is localized to insulin granules in live β-cells. MIN6 cells were transfected with phogrin-mCherry to label insulin granules (A and B, red), TfR-mCherry to label the recycling endosomes (C, red), ST-mRFP to label the TGN (E, red), or incubated overnight with rhodamine (Rh)-dextran to label the lysosomes (D, red). Cells were then labeled with BODIPY-chol (green in all panels) for 3 h at 37 °C and imaged live by confocal microscopy. The top row in each panel shows individual confocal planes; the bottom row shows projections of all confocal planes in a z-stack. (B) Three examples of super-resolution SIM images of phogrin-mCherry and BODIPY-chol localized to the same granule. The white scale bar, 10 μm, applies to A, C, D, E; Black scale bar for B: 0.5 μm.