Bithionol and hexachlorophene inhibit SV40 replication in CV1 monkey kidney cells. A. SV40 replication was assessed in the presence of MAL2-11B, bithionol (“Bith”) or hexachlorophene (“Hexa”) for 2 days, after which the replication-competent virus was titred by plaque assay. The data represent three separate infections, each measured in at least two technical replicates, +/− SD. Data were standardized relative to the DMSO control, which was set at “100%.” B. Replication of viral DNA in a parallel experiment was quantified by qPCR under identical conditions to those used in part A. The data represent the means of 12 independent experiments, +/− SE. Please note that in A and B MAL2-11B was used at a final concentration of 100 μM, whereas bithionol and hexachlorophene were used at a final concentration of 30 μM.