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. Author manuscript; available in PMC: 2013 Oct 15.
Published in final edited form as: Transplantation. 2012 Oct 15;94(7):687–694. doi: 10.1097/TP.0b013e3182633478

FIGURE 3.

FIGURE 3

Up-regulated expression of TNF-α and IL-1β genes in the mediation of small size graft injury. Increased TNF-α (A) and IL-1β (B) gene expression was demonstrated at an early phase (2–3 days) after SSGLT, and MnTBAP significantly attenuated the enhanced TNF-α and IL-1β gene expression as demonstrated by real time RT-PCR analysis in the graft tissue. The GAPDH gene was used as a house-keeping control. n=6, *, p<0.05, **, p<0.01 compared to the SSGLT group. The sequences of the forward and reverse primers are: 5′-CGA TTT GCC ATT TCA TAC CAG-3′ and 5′-AGT ACT TGG GCA GGT TGAC-3′ for TNF-α (amplicon size: 180 bp); 5′-TGT GGA TCC CAA ACA ATA CCC-3′ and 5′-TAT GTC CCG ACC ATT GC-3′ for IL-1β (amplicon size: 175 bp); 5′-TGT GCA GTG CCA GCC TCG TCT-3′ and 5′-TTG CCG TGG GTA GAG TCA TAC-3′ for GAPDH (amplicon size: 190 bp).