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. Author manuscript; available in PMC: 2013 Nov 1.
Published in final edited form as: Biochim Biophys Acta. 2012 Jun 15;1823(11):2079–2086. doi: 10.1016/j.bbamcr.2012.06.004

Figure 1. Physical characterization of Ub-AML by LC-MS.

Figure 1

The modifiied protein was analyzed by RP-HPLC (Restek Viva C18 column, 3 micron particle size, 2.1 × 150mm). The column was equilibrated in 30% AcN/0.05% TFA at 0.2 mL/min and developed with a linear gradient to 50% AcN/0.05% TFA over 11 min. The absorbance at 214 nm (upper panel, black line) of the effluent from the column was recorded prior to injection into the ionization chamber of an ESI/ToF mass spectrometer. The total ion chromatogram is shown as the dark gray line in the upper panel. The mass spectrum (lower left panel) was extracted for the peak eluting at ~7 min and deconvoluted using the program Max Entropy to derive the parent mass spectrum shown in the lower right panel.