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. Author manuscript; available in PMC: 2013 Nov 1.
Published in final edited form as: Biochim Biophys Acta. 2012 Jun 15;1823(11):2079–2086. doi: 10.1016/j.bbamcr.2012.06.004

Figure 2. Monitored progression of luminescence for Ub-AML and Z-RLRGG-Glo.

Figure 2

Increasing concentrations (indicated) of UCHL3 were incubated with either 500nM Ub-AML (A) or 20μM Z-RLRGG-AML(C) with immediate and continuous monitoring of luminescence (RLUs). Calculated S/B values (see methods) are shown for Ub-AML (B) and Z-RLRGG-AML(D), with the dashed line drawn to illustrate S/B = 3. Data are representative of triplicate measurements.