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. Author manuscript; available in PMC: 2013 Nov 1.
Published in final edited form as: Biochim Biophys Acta. 2012 Jun 15;1823(11):2079–2086. doi: 10.1016/j.bbamcr.2012.06.004

Figure 3. Determination of assay dynamic range for various DUB substrates.

Figure 3

Panel A: Indicated concentrations of UCHL3 (▲/△), USP2core (◆/◇), USP7 (●/○) and USP8 (■/□) were incubated with either 250nM Ub-AML (closed symbols) or 20μM Z-RLRGG-AML (open symbols) for 30min prior to RLU measurement and S/B calculation (see methods). Panel B: Indicated concentrations of USP2core (◆/◇), USP7(●/○), and USP8 (■/□) were incubated with 250nM of either Ub-AMC (closed symbols) or Ub-Rho110 (open symbols) with immediate and continuous monitored fluorescence. S/B values were calculated from 30m time points (see methods). Data are representative of triplicate measurements.