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. Author manuscript; available in PMC: 2013 Nov 1.
Published in final edited form as: Biochim Biophys Acta. 2012 Jun 15;1823(11):2079–2086. doi: 10.1016/j.bbamcr.2012.06.004

Figure 5. The use of hSUMO2- and NEDD8-AML for measuring isopeptidase activity.

Figure 5

Panel A: Indicated isopeptidases (50nM to 70pM) were incubated with hSUMO2-AML (250nM, black bars) or Z-RLRGG-AML (20μM, white bars) for 30m prior to RLU measurement and S/B calculation according to methods. At 200pM isopeptidase (shown) hSUMO2-AML displays clear selectivity for desumoylases over DUBs. Errors bars indicate S.D. from triplicate measurements. Panel B: RLUs were measured from NEDD8-AML (250nM, black bars) or Z-RLRGG-AML (20μM, white bars) after 30m incubation in either the presence or absence of 500pM Den1.