Table 2.
Summary of the LC–MS analysis result of the protein collected from the urine of IgAN patients and healthy donors by IP method using anti-IgA conjugated beads and BSA beads
| Beads: | anti-IgA conjugated beads | BSA beads | ||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Disease: | IgAN | Other kidney diseases | IgAN | |||||||||||||
| Sample no: | 1 | 2 | 3 | 4 | 10 | 11 | 12 | 5 | 6 | 7 | 8 | 9 | 1 | 2 | ||
| ID | Protein name | |||||||||||||||
| Cell component or other | gi|340166 | Uromodulin | 3 | 3 | 1 | 3 | 1 | 1 | 1 | |||||||
| gi68838 | Aquaporin | 1 | 1 | |||||||||||||
| gi|7331218 | Keratin 1 | 2 | 2 | 2 | 1 | 2 | 1 | 2 | 1 | 2 | ||||||
| gi|34073 | Cytokeratin 4 (408 AA) | 1 | 1 | 1 | 1 | |||||||||||
| gi186629 | Keratin 10 | 1 | 1 | 1 | ||||||||||||
| gi|34033 | Keratin 13 | 1 | 1 | |||||||||||||
| gi177139 | Keratin 14 | 1 | 1 | 1 | 1 | |||||||||||
| gi186685 | Keratin 16 | 1 | 1 | 1 | ||||||||||||
| gi34081 | Keratin 17 | 1 | ||||||||||||||
| Serum protein | gi|4557871 | Transferrin | 14 | 14 | 1 | 1 | 1 | |||||||||
| gi|28592 | Serum albumin | 3 | 45 | 6 | 2 | 4 | 3 | 2 | 1 | 5 | 3 | 3 | ||||
| gi|4557385 | Complement component 3 (C3) | 1 | 3 | 1 | ||||||||||||
| gi|306882 | Haptoglobin precursor | 2 | 3 | |||||||||||||
| gi|72059 | Leucine-rich alpha-2-glycoprotein | 1 | 2 | 2 | ||||||||||||
| gi177827 | Alpha-1-antitrypsin | 1 | 2 | 2 | 2 | 1 | 2 | |||||||||
| gi45067732 | S100 calcium-binding protein A9 | 1 | 2 | |||||||||||||
| gi|493852 | Hemoglobin | 5 | 1 | 1 | 1 | 8 | 2 | |||||||||
| gi|224053 | Macroglobulin alpha2 | 1 | 2 | |||||||||||||
| Antibody component | gi|223099 | IgA alpha1 Bur | 2 | 1 | ||||||||||||
| gi|223335 | Ig kappa L I Den | 1 | 1 | |||||||||||||
| gi|229528 | Protein Len, Bence-Jones | 2 | 3 | 1 | ||||||||||||
| gi33700 | Ig lambda light chain | 1 | 2 | 1 | 1 | 1 | 1 | |||||||||
| gi9857759 | IgG4 heavy chain | 1 | ||||||||||||||
| gi229526 | Protein Rei, Bence-Jones | 3 | 5 | |||||||||||||
| Ig kappa light chain | 3 | 3 | 2 | |||||||||||||
| Ig heavy chain | 2 | 4 | 2 | 1 | ||||||||||||
Urine samples were from IgAN patients (1, 2, 3, 4, 10, 11, 12), amyloidosis (5), SLE (6), DMN (7, 8), and MCNS (9). The numbers in the column show the identified number of fragments by LC–MS analysis