Fig. 4.

NF-κB, c-Jun and CREB are involved in LPC-elicited COX-2 protein upregulation. 48 h after transfection with p65, c-Jun, CREB or scrambled control siRNA (control), EA.hy 926 cells were treated with 16:0- or 20:4-LPC for 16 and 8 h, respectively. Subsequently, protein levels of (A) p65, c-Jun and CREB as well as of (B) COX-2 ere analyzed by Western blot as described in Section 2. Representative images are presented. COX-2 densitometric values are normalized to β-actin and expressed as fold-induction, setting the value of cells transfected with control siRNA and treated with LPCs as 1. Bars represent the mean ± SD (n = 6). (*, **, *** indicate significant difference between LPC treated cells transfected with specific or control siRNA).