Figure 5.

Integrins Regulate Spindle Orientation and Localization of Asymmetric Proteins

(A and B) 3D reconstruction of confocal projections of mitotic esg-positive ISCs (green) labeled by pH3 (red) in wild-type (A) and mew (B) knockdown cells. Basement membrane is labeled by Laminin (red).

(C–E) Quantification of angles of spindle orientation away from the basement membrane upon knockdown of mew (n = 14), mys (n = 6), if (n = 12), and apc (n = 7) (C) cells. Overexpression of Tor^D/β_cyt (n = 14) and FAK^56wt (n = 9) dominant-negative adhesion constructs in shown in (D) and knockdowns of Par complex members Par-6 (n = 21), Baz (n = 7), and aPKC (n = 8) is shown in (E).

Statistical significance was determined by Wilcoxon rank sum test. Scale bars = 5 μm. See also Figure S5.