Figure 4.

Epsin ENTH Domain Causes Extensive Membrane Vesiculation

(A) Epsin (10 μM) incubated with 0.125 mg/ml Folch liposomes for 1 hr at room temperature. Samples for electron microscopy were taken before and after centrifugation. For sonicated liposomes a sample was subjected to probe sonication for 5 s. Samples for centrifugation were spun as indicated in the diagram. Pellets (P) were resuspended in the same volume of buffer as the supernatant (S). Lipids and proteins were visualized as described in Experimental Procedures.

(B) Folch liposomes filtered to various sizes were subjected to centrifugation and the lipid distribution was assessed by SDS-PAGE. Samples for electron microscopy were taken before the spin. A more complete distribution of vesicle sizes is shown in Figure S4C.

See also Figure S4.