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. Author manuscript; available in PMC: 2014 Mar 1.
Published in final edited form as: Mucosal Immunol. 2012 Jul 4;6(2):235–243. doi: 10.1038/mi.2012.63

Figure 1. Chlamydia infection of human trophoblast cell lines leads to induction of IL-1β expression and IL-1β processing.

Figure 1

Trophoblast cells were either non-infected (NI) or infected with Chlamydia (Ct), after which supernatants were collected and either RNA or protein extracted from cells. (a) Ct infection of HTR8 cells significantly induced IL-1β mRNA expression after 24hrs as determined by qRT-PCR; and (b) significantly induced IL-1β secretion after 48hrs as determined by ELISA. (c) Ct infection of Sw.71 cells significantly induced IL-1β secretion after 36hrs (n=3; *p<0.05 relative to the NI control). Cell lysates from (d) HTR8 and (e) Sw.71 cells after either NI or infection with Ct were evaluated for pro-IL-1β (31kDa), active IL-1β (17kDa) and active caspase-1 (20kDa) expression by Western blot (representative blots are shown). Barcharts show quantification of protein expression as determined by densitometry and normalized to β-actin (n=3; *p<0.05 relative to the NI control). (f) Cell supernatants from Sw.71 cells either NI or infected with Ct for 36hrs were evaluated for pro- and active IL-1β and active caspase-1 expression by Western blot. Barcharts show quantification of protein expression as determined by densitometry and normalized to β-actin (n=4; *p<0.05 relative to the NI control).

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