Fig. 1.

In situ monitoring of plasmid transfer in a biofilm cultivated in a continuous flow cell system irrigated with 200 mg/L (a) or 20 mg/L (b) concentrations of glucose, respectively. Spatial distribution of green transconjugants (P. putida KT2442 with TOL-gfpmut3b plasmid, green, regions between blue or red boundaries, marked as white or red arrows on y–z plane), donors (P. putida TUM-PP12 with TOL-gfpmut3b plasmid, yellow, region between red and blue boundaries), recipients (P. putida KT2442, region between white and blue boundaries, colorless of the reflection image) in this view, and segregants (P. putida TUM-PP12 (miniTn5Putdsred), red). Red arrows point to regions of transconjugants on x–z plane and black arrow points to cells bedded inside the inner cone. The micrograph is displayed in an orthogonal view to show the biofilm structure in three sets of 2-D sections; xy, xz, and yz. Bar size 20 μm