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. Author manuscript; available in PMC: 2013 Jan 5.
Published in final edited form as: Appl Microbiol Biotechnol. 2012 Jun 6;97(1):317–328. doi: 10.1007/s00253-012-4179-9

Fig. 3.

Fig. 3

In situ monitoring of plasmid transfer in a biofilm cultivated in a continuous flow cell system cultivated on FAB medium containing concentrations of 0 (control, no antibiotics, a and d), 0.5 μg/ml kanamycin (b and e) or 0.1 μg/ml imipenem (c and f), respectively. Spatial distribution of green transconjugants (P. putida KT2442 with TOL-gfpmut3b plasmid, green, marked as white arrow), donor (P. putida TUM-PP12 with TOL-gfpmut3b plasmid, yellow) and segregants (P. putida TUM-PP12 (miniTn5Putdsred), red). Recipients (P. putida KT2442) are colorless cells (of reflection images) in this view. The micrograph is displayed in an orthogonal view to show the biofilm structure in three sets of 2-D sections; xy, xz, and yz. A–c Images monitored on day 6. c–e Matured biofilms formed on day 8. Bar size 20 μm

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