Fig. 6.

Colony PCR analyses of transconjugants, donors, segregants, and recipient cells obtained by plating sorted cells from flow cytometry equipped with FACS machine on LB plates. a1, 2 Positive control, using donor cells (dual labeling) and segregational lost cells (DsRed) as DNA templates; 3, 4 negative control, using recipient cells (no color) and diH2O as DNA templates; 5, 6, 7, 8, 9 using Transconjugants as DNA templates; 10, 11, 12, 13, 14 using segregational lost cells as DNA templates; S standard DNA HyperLadder I from Bioline. Both DsRed and GFP specific primers used for all PCR reactions. Arrow points to a false reading of a donor cell as a transconjugant cell