Table 2.
Effect of substrate concentrations on biofilm struc- tures of P. putida KT2442 using cluster mapping
| Substrate glucose concentration (mg/L) | 200 | 20 |
|---|---|---|
| Angle of cone (θ), degree | 56.89 a | 44.69 b |
| Outer layer thickness L (μm) | 21.82±3.98 (4.46±1.23) | 12.54±2.55 (5.21±0.87) |
| Donors (transconjugants) | ||
| Inner layer thickness L (μm) | – | 5.34±1.58 (2.12±0.98) |
| Donors (transconjugants) | ||
| Volume of transconjugants VT (μm3) | 1.27E+04 | 7.37E+03 |
| Volume of donors VD (μm3) | 1.12E+05 | 3.15E+04 |
| Transfer frequencies (VT/VD) | (1.13E-01) a | (2.34E-01) b |
Values with different letters differ signifi- cantly (P<0.05) within row