. Author manuscript; available in PMC: 2013 Jan 5.

Published in final edited form as: Appl Microbiol Biotechnol. 2012 Jun 6;97(1):317–328. doi: 10.1007/s00253-012-4179-9

Table 3.

Effect of substrate concentrations on P. putida KT2442 bio- film structure analysis using COMSTAT

Substrate glucose concentration (mg/L)	Roughness coefficient	Surface area to volume ratio (μm²·μm⁻³)
20	1.78±0.09	7.37±0.73
200	0.74±0.06	2.57±0.38

Roughness coefficient= $= \frac{1}{N} \sum_{i = 1}^{N} \frac{∣ L_{ft} - {\bar{L}}_{f} ∣}{{\bar{L}}_{f}}$ , where L_fi is the ith individual thickness measurement, ${\bar{L}}_{f}$ is the mean thickness, and N is the number of thickness measurements

Surface area to volume ratio=surface area/biovolume