Table 4.
Enumeration of conjugative transfer frequency (T/D) of TOL-gfpmut3b plasmid from P. putida TUM-PP12 to P. putida KT2442 by CFU, FACS, and CLSM
| Methods | Transconjugants (cells/cm2) | Transfer frequency (T/D) | Transfer frequency standard deviation |
|---|---|---|---|
| CFUa | (9.73×104)a | 0.82×10−1 | 2.16×10−2 |
| FACSb | (1.26×106)b | 3.30×10−1 | 4.23×10−2 |
| CLSMc | (4.07×106)c | 1.24×10−1 | 3.06×10−2 |
Values with different letters differ significantly (P<0.05) within column
a
CFU colony forming unit. Enumeration of cells by plating assorted destructive biofilms on plates
b
FACS fluorescent activated cell sorting. Flow cytometry enumeration of cells by using the defined gates. Four populations of D, T, R, and S in biofilms can be isolated by FACS
c
CLSM confocal laser scanning microscope. Estimation of D and R directly by using CLSM microscopy combined with quantification method of image analysis