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. 1982 Jul;79(13):4025–4029. doi: 10.1073/pnas.79.13.4025

Purification of polyoma virus medium-size tumor antigen by immunoaffinity chromatography.

G Walter, M A Hutchinson, T Hunter, W Eckhart
PMCID: PMC346569  PMID: 6287461

Abstract

We have used antibodies against the synthetic peptide Lys-Arg-Ser-Arg-His-Phe, corresponding to the six COOH-terminal amino acids of the polyoma virus medium tumor (T) antigen, to purify the medium T antigen by affinity chromatography. Release of the medium T antigen from the anti-peptide antibody was achieved under mild conditions by using a large excess of the peptide in an isotonic buffer at neutral pH containing mixed detergents. This procedure yielded a 2,500-fold purification of the medium T antigen in a single step. The protein kinase activity associated with the medium T antigen was also released and was studied in this active state in solution. Sedimentation analysis showed that the bulk of the purified medium T antigen was in a monomeric form (Mr about 42,000) not associated with protein kinase activity. A small fraction of the medium T antigen was found in a rapidly sedimenting form (Mr about 200,000) that possessed protein kinase activity.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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