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. 2012 Oct 1;26(19):2138–2143. doi: 10.1101/gad.197582.112

Figure 5.

Figure 5.

YAP/TAZ mediate physiological functions of PARs in gene induction, cell migration, and invasion. (A) YAP/TAZ knockdown by siRNAs in MCF10A cells (left) and MDA-MB-231 cells (right). Cells were transfected with the indicated siRNAs. The protein levels were determined by immunoblotting. (B,C) MDA-MB-231 cells were transfected with the indicated siRNAs and serum-starved for 12 h. (B) After treating with 2 μM TRAP6 for 4 h, mRNA levels of CTGF, Cyr61, CXCL1, END1, FSTL, INHBA, and PTGS were measured by quantitative PCR. (C) Protein levels of YAP/TAZ, CTGF, and Cyr61 were determined by immunoblotting. (D) MCF10A cells were transiently transfected with the indicated siRNAs. Cell migration was determined by transwell cell migration assay. Cells were stained with crystal violet (top panel) and quantified (bottom panel). (E) MDA-MB-231 cells were transiently transfected with the indicated siRNAs. Cell invasion was determined by Matrigel invasion assay.