Skip to main content
. 2012 Oct 1;26(19):2192–2205. doi: 10.1101/gad.192666.112

Figure 2.

Figure 2.

Med23 deficiency up-regulates cytoskeleton genes via the RhoA/MAL pathway. (A) Stable MEF cell lines were generated by retroviral expression of siMed23 or siCtrl. After puromycin selection, the knockdown efficiency was analyzed by Western blot. (B) siCtrl and siMed23 MEFs were serum-starved or serum-stimulated with or without LatB pretreatment. The expression of Vcl and Srf was examined by real-time PCR and normalized to Gapdh level. (C) Med23 knockout (KO) MEFs were infected with retroviruses expressing siMal (KO+siMal). After serum starvation or stimulation as in Figure 1C, total RNA was extracted and subjected to real-time analysis of Acta2, Actg2, and Sm22a expression. Their expression was normalized to Gapdh. (D) siMed23 MEFs were infected with retroviruses expressing siMal (siMed23+siMal). The expression of cytoskeletal genes (Vcl, Srf, Acta2, and Actg2) was analyzed in serum-stimulated siCtrl, siMed23, and siMed23+siMal MEFs by real-time PCR. The expression was normalized to Gapdh.