Figure 5.

Active zone proteins SYD-1 and SYD-2 are necessary for MIG-10B synaptic localization. (A–C) Confocal micrographs showing MIG-10B∷GFP subcellular localization expressed cell-specifically in AIY in wild-type (A) and syd-1(ju82) (B) and syd-2(ju37) (C) mutant animals. Note that MIG-10B∷GFP is less enriched in zone 2 and is more diffuse in zone 3 in the two mutant backgrounds as compared with wild-type animals. (D) Schematic of the Rac GTPase and active zone protein pathways converging on MIG-10B to instruct MIG-10B localization and synaptic vesicle clustering. In all images, the asterisk (*) represents the location of the cell body. Bar: A, 5 μm (applies to A–C).