Figure 3.

Flow cytometric analysis with AS1411-cotinine/anti-cotinine antibody complexes. (A) Raji cells were incubated with complexes containing the indicated concentrations of AS1411-cotinine and 100 nM anti-cotinine antibody and subsequently stained with FITC-conjugated anti-human Fc antibody. As a control, CRO26-cotinine and palivizumab were used in place of AS1411-cotinine and anti-cotinine antibody, respectively. Control signal was obtained from cells incubated only with FITC-conjugated anti-human Fc antibody. (B) Raji, HepG2, U87MG, and NIH3T3 cells were incubated with AS1411-cotinine/anti-cotinine antibody complexes and subsequently stained with FITC-conjugated anti-human Fc antibody.