Figure 5.
Enzyme immunoassay analysis using pegaptanib-cotinine/anti-cotinine antibody complexes. Pegaptanib-cotinine/anti-cotinine antibody complexes were allowed to react with VEGF165 immobilized on a microtiter plate. The concentration of anti-cotinine antibody was maintained as one-half that of pegaptanib-cotinine as the antibody is bivalent. Bevacizumab was used as a positive control. HRP-conjugated rabbit anti-human IgG antibody and 3,3',5,5'-tetramethylbenzidine substrate solution were employed to determine the amount of complex bound to the microtiter plate. The results are expressed as the mean ± standard deviation of triplicate measurements.