Figure 5.

Plant odorants do not interfere with the binding of Z11-16:Ald to HvirPBP2. Competitive fluorescence binding assays were performed, employing HvirPBP2 and 1-NPN, both at 2 μM concentration in Ringer solution. The maximum emission of 1-NPN fluorescence was monitored after increasing concentrations (0–10 μM) of Z11-16:Ald were added and the plant odorants indicated (1:1 ratio). Maximum fluorescence intensities over concentration are shown as percentages of the value in the absence of the mixture. For comparison, the displacement curve determined for the pheromone component alone is depicted in addition to the displacement curves for the mixtures.