Fig. 4.

Neighbour-joining consensus tree of partial 18S rRNA gene sequences of 13 species of ciliates found within Brown Band Disease and Progressive White Syndrome. Number in brackets relates to number of sequences retrieved from single cell isolates. Sequences were aligned in clustal w2 (Larkin et al., 2007), using an IUB cost matrix with a gap open cost of 15 and a gap extend cost of 7. A neighbour-joining consensus tree (1000× re-sampling) was constructed in Geneious Pro 5.0 using the Tamura genetic distance model (Tamura, 1994) with an opalinid protist, Opalina ranarium (AF141970), as the outgroup.