Table 1.
Organelle marker content of phagosomes containing A. felis or zymosan analyzed by confocal immunofluorescence microscopy
| Marker protein | Organelle | Phagosomes containing
|
|
|---|---|---|---|
| A. felis (%) | Zymosan (%) | ||
| rab5 | EE | 4 ± 4 (444) | 7 ± 7 (322) |
| EEA1 | EE | 2 ± 3 (447) | 3 ± 2 (169) |
| Transferrin receptor | EE | 4 ± 2 (321) | 9 ± 4 (281) |
| TACO | EE/PM* | 4 ± 2 (242) | ND |
| VAMP-8 | EE/LE | 36 ± 11 (672) | 72 ± 12 (352) |
| rab7 | LE | 4 ± 3 (292) | ND |
| CD-M6PR | Golgi/LE | 1 ± 1 (482) | 5 ± 6 (220) |
| LAMP-1 | LE/lys | 26 ± 2 (255) | 84 ± 9 (332) |
| LAMP-2 | LE/lys | 29 ± 4 (158) | 89 ± 12 (254) |
| β-Glucuronidase | lys | 33 ± 12 (614) | 77 ± 11 (242) |
| Calnexin | ER | 4 ± 2 (400) | 9 ± 4 (236) |
J774E cells were infected for 1 h, chased for 2 h, and processed with the respective antibodies as described in Materials and Methods. Analysis was by confocal immunofluorescence microscopy. Data indicate percentage of phagosomes positive for the respective marker protein and are from 3 to 10 independent experiments. Mean and SDs and number of phagosomes counted (in parentheses) are indicated.
EE, early endosome; LE, late endosome; lys, lysosome; PM, plasma membrane; ND, not determined.
*
Predominantly PM and early phagosomes (27).