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Colocalization of VGluT2 mRNA and PTH2R mRNA in the anterior hypothalamus as detected by fluorescent in situ hybridization. (A) VGluT2 mRNA (red puncta) over cell nuclei (blue, DAPI). (B) PTH2R mRNA (green puncta) over cell nuclei (blue, DAPI). (C) a merged image, where the arrows point to cells expressing both PTH2R/VGluT2 mRNAs and the arrowhead points to a neuron with only VGluT2 signal. Scale bar = 10 μm.
