Table 1.
Embryonic developmental events of S. sphaeroides. Three replicate fertilization experiments were conducted and for each developmental stage, 10 embryos from each replicate were used for the observation and measurement of embryos.
Time after insemination | Developmental stages | Diameter (μm) |
---|---|---|
00.01 h | Fertilized eggs with the formation of fertilization membrane | 134.86 ± 5.35 |
00.05 h | Fertilized eggs with complete fertilization membrane | 134.86 ± 5.35 |
01.12 h | 2-cell stage | 154.27 ± 7.17 |
02.03 h | 4-cell stage | 157.14 ± 5.84 |
02.28 h | 8-cell stage | 159.25 ± 6.29 |
02.51 h | 16-cell stage | 161.10 ± 5.80 |
03.12 h | 32-cell stage | 163.65 ± 4.78 |
03.32 h | Multicell (Morulla) stage | 164.38 ± 4.48 |
08.45 h | Hatching Blastula | 174.72 ± 4.43 |