Table 2.

Electrophysiological properties of layer 2/3 pyramidal cells expressing GCaMP3 and GECOs.

Ephys	GC3 (n = 13)	1.0 (n = 10)	1.1 (n = 10)	1.2 (n = 9)	R (n = 9)	GEM (n = 9)	no exp (n = 11)
Vm a	−80 ± 5.8	−81 ± 4.8	−82 ± 5.6	−82 ± 4.1	−78 ± 2.1	−80 ± 3.8	−82 ± 8.7
Rm b	135 ± 32	107 ± 44	124 ± 54	134 ± 66	128 ± 25	113 ± 31	139 ± 42
AP amplitude c	100 ± 6.8	98 ± 9.2	103 ± 8.1	101 ± 8.0	105 ± 7.1	100 ± 4.9	107 ± 19
AP threshold d	−43 ± 5.5	−41 ± 5.1*	−45 ± 3.6	−42 ± 3.7	−43 ± 7.7	−37 ± 5.5***	−47 ± 7.4
AP half width e	2.0 ± 0.8	2.8 ± 1.1**	2.0 ± 0.6	2.1 ± 0.6	2.2 ± 1.4	4.2 ± 2.7***	1.7 ± 0.5

All values are corrected for liquid junction potential (12 mV). Significant difference in Dunn's post-hoc test comparing GECI-expressing cells and wild-type cells:

^*p < 0.05;

^**p < 0.01;

^***p < 0.001. GC3, GCaMP3; 1.0, G-GECO1.0; 1.1, G-GECO1.1; 1.2, G-GECO1.2; R, R-GECO1; GEM, GEM-GECO1; no exp, cells from wild-type animals without OGB-1.

^aResting membrane potential (mV).

^bInput resistance (MΩ).

^cAmplitude of action potential measured from the resting membrane potential (mV).

^dThreshold voltage for action potential generation defined as the point where the first temporal derivative of the voltage first exceeds 50 mV/ms (mV).

^eFull width of action potential measured at half the amplitude (ms).