Table 3.

SNR of GCaMP3 and GECOs in cortical layer 2/3 pyramidal cells.

Number of APs	GC3 (n = 13)	1.0 (n = 10)	1.1 (n = 10)	1.2 (n = 9)	R (n = 9)	GEM (n = 9)
1	1.5 ± 0.8	1.4 ± 0.2	1.2 ± 0.4	1.1 ± 0.2	1.4 ± 0.9	0.9 ± 0.1**
2	2.5 ± 1.6	2.4 ± 1.6	1.7 ± 0.5	1.4 ± 0.4*	1.6 ± 0.9*	1.0 ± 0.2***
5	7.2 ± 6.0	4.8 ± 3.2	4.1 ± 1.8	2.8 ± 1.2*	2.4 ± 1.1**	1.4 ± 0.3***
10	15 ± 12	8.3 ± 5.5	9.0 ± 4.9	5.8 ± 2.9*	3.2 ± 1.2***	2.1 ± 0.8***
20	27 ± 22	15 ± 12	17 ± 9.2	10 ± 6.5*	4.8 ± 2.1***	3.4 ± 1.0***
40	157 ± 39	103 ± 70	59 ± 12*	73 ± 39	11 ± 3.1***	11 ± 3.2***

Action potentials (APs) were evoked at 20 Hz except for 40 APs evoked at 83 Hz. The numbers of recorded cells in the table apply to 1–20 APs; for 40 APs, n = 8 for GCaMP3 and R-GECO1, n = 7 for G-GECO1.0, and n = 9 for G-GECO1.1, G-GECO1.2 and GEM-GECO1. Significant difference in Dunn's post-hoc test comparing GCaMP3-expressing cells and GECO-expressing cells:

^*p < 0.05,

^**p < 0.01,

^***p < 0.001.