Fig. 6.

LA reduces AdPLA levels and PGE₂ secretion and increases intracellular cAMP levels in 3T3-L1 adipocytes. A: AdPLA protein levels at 1 and 24 h of treatment with LA (250 μM). B: PGE₂ released to the media in 3T3-L1 adipocytes treated with LA (250 µM) for 24 h. C: Intracellular cAMP levels at 1 and 24 h of treatment with LA (250 μM). D: Effects of PGE₂ (0.5 ng/ml) on the lipolytic action of LA (250 μM) in the presence or absence of the EP3 antagonist L78106 (10 μM). Data are expressed as mean ± SE of at least three independent experiments. *P < 0.05, **P < 0.01, and ***P < 0.01 vs. control (vehicle-treated cells). ^#P < 0.05 vs. PGE2-treated cells. ^aP < 0.05 vs. basal LA-treated cells.