Figure 3.

The Tor proteins regulate Pol I/III transcription through Gln3p and Nil1p. Strains PM38 (wild type, wt), PM71 (gln3Δ), MS221 (nil1Δ), PH2 (ure2Δ), and P40-1A (gln3Δ ure2Δ) were treated with 50 nM rapamycin for 30 min and then labeled with tritiated adenine for another 30 min. After the second 30-min period, total RNA was trichloroacetic acid precipitated and counted. Fold translation remaining is defined as counts in the rapamycin-treated samples divided by counts in the untreated samples, normalized for cell density. Error bars span 1 SD about the mean for at least three experiments performed.