Fig. 6.

Analysis of the nuclear receptor activational capacity of sterols and oxysterols. Analysis of luciferase activity in SN4741 cells (mouse neuronal cell line) transfected with an LXR-responsive luciferase reporter construct (LXRE) and LXRα or -β and stimulated for 24 h with desmosterol (10 μm) or the compounds indicated, all at 10 μM, i.e., (24E),26-hydroxydesmosterol; (24Z),26-hydroxydesmosterol; 24-oxocholesterol; and 22-oxocholesterol. The firefly luciferase activity was normalized to Renilla luciferase activity, and the values are expressed as fold activation over the normalized basal LXR response element-luciferase activity set to 1. Data are means ± SE (n = 3); *P < 0.05, compared with vehicle treatment.