Figure 4.  .

CCL21 or CXCL10 increases transmigration of T. gondii tachyzoite–infected human dendritic cells across simulated human retinal vascular endothelium. Graphs showing the number of dendritic cells recovered from lower chambers of transwells divided by human retinal endothelial cell monolayers with (A, B) CCL21 or (C, D) CXCL10, or no chemokine added to lower chambers at commencement of the experiment. Prior to use in assays, dendritic cells were (A, C) exposed to RH strain T. gondii tachyzoites or (B, D) remained unexposed. Bars: mean; error bars: SEM; n = 4 to 8 wells; P values by Student's t-test, two-tailed; NS, no significant difference. Graphs presented in (A), (C), and (D) show results that are representative of those obtained in 2 to 3 independent experiments. Graph presented in (B) shows results that are representative of those obtained in 2 of 4 independent experiments.