FIG. 6.

PCR to assess parasite load in internal organs of infected mice in a mild climate. Amplicons were resolved in 2.0% agarose gels. Even numbers correspond to infected mice harboring the M379 strain after 6 months of evolution. Odd numbers correspond to healthy mice of the same age (1, molecular weight; 2, positive control M379 axenic culture; 3, tissue culture negative control; 4 and 5, footpad; 6 and 7, liver; 8 and 9, spleen; 10 and 11, pancreas; 12 and 13, kidney). The experiments were performed in triplicate. More than 10 infected mice were analyzed with similar results.