Figure 2. .
Effect of depletion of ERK2 on histamine-stimulated rat goblet cell high molecular weight glycoconjugate secretion. Cultured rat goblet cells were preincubated for 48 hours with no additions, siRNA for ERK2 (ERK siRNA), or siRNA for a scrambled sequence (scRNA), and then stimulated with histamine (His) at 10−6 and 10−5 M for 2 hours. High molecular weight glycoconjugate secretion was measured by enzyme-linked lectin assay. Data are mean ± SEM of three independent experiments. *Indicates statistical significance from no addition; #indicates statistical significance from histamine alone.