Figure 4. .
Effect of a phospholipase C inhibitor on intracellular [Ca2+] ([Ca2+]i) stimulated by histamine in rat conjunctival goblet cells. Cultured rat goblet cells were loaded with the Ca2+ indicator dye fura 2 and pre-incubated for 30 minutes with the phospholipase C inhibitor U73122. The [Ca2+]i was visualized in single goblet cells by pseudocolor as indicated in (A). The top four panels indicate the [Ca2+]i response to histamine (His, 10−6 M) at 0, 30, 50, and 120 seconds. The bottom four panels indicate the [Ca2+]i response to histamine (10−6 M) at 0, 30, 50, and 120 seconds after pre-incubation with U73122 (10−8 M). Traces shown in (B) are mean of four individual experiments. The percent inhibition of peak [Ca2+]i was calculated and shown as mean ± SEM in (C). #Indicates statistical significance from histamine alone.