TABLE I. Lateral Mobility of Plasma Membrane Lipids in B16 Cells3.
| Cell Type |
FAST-DiO1 | DiO2 | ||
|---|---|---|---|---|
| Diffusion (×10−10cm2/sec) |
Mobile Fr. (%) |
Diffusion (×10−10cm2/sec) |
Mobile Fr. (%) |
|
| B16 | 8.39±5.51 (n=13) |
89.3±10.5 (n=13) |
7.77±0.8 (n=15) |
80.03±3.8 (n=15) |
| GM95 | 13.1±8.39 (n=25) |
87.8±11.1 (n=25) |
9.47±2.50 (n=7) |
70.07±10.2 (n=7) |
| NIH3T3 | 13.8±0.8.98 (n=15) |
84.3±12.8 (n=15) |
10.89±3.16 (n=7) |
75.59±19.17 (n=7) |
1
Lateral diffusion was measured by monitoring FRAP of Fast-DiO-labeled cells (see Methods section)
2
Lateral diffusion was measured by monitoring FRAP of DiO-labeled cells (see Methods section)
3
n=number of cells (measurements) within a single set of experiment and values are expressed as ±SEM.