Table II.

Summary of in vitro apoptosis detected in LCs from WT and Bid KO mice.

Exp # a	% Specific LC loss b		% Specific Apoptosis c		% Specific LC Death
	WTd	Bid KO	WT	Bd KO	WT	Bd KO
1	46.7	0.5	21.0	2.0	67.7	2.5
2	47.4	5.0	12.7	3.0	60.1	8.0
3	30.4	7.4	16.0	0.0	46.4	7.4
Average	41.5	4.3	16.6	1.7	58.1	6.0
St Dev	9.6	3.5	4.2	1.5	10.8	3.0
P <e		0.02		0.02		0.01

^aResults from three independent experiments are shown. cDCs were gated for each experiment as follows: Exp # 1, I-A; Exp # 2, CD11c; Exp # 3, Langerin (CD207). WT or Bid KO LCs comprises the majority of cDCs isolated from ear skin cultures, as described in Materials and Methods.

^b% Specific LC loss is calculated as the difference in percent of LCs remaining after overnight culture in the presence of OVA antigen and relevant OT-2 versus irrelevant 3A9 naïve purified CD4 T cells .

^c% Specific Apoptosis is calculated as the difference in apoptosis detected by annexin V and/or 7-AAD staining of remaining DCs after overnight culture in the presence of OT-2 or 3A9 T cells.

^d% Specific LC Death = % Specific LC loss + % Specific Apoptosis.

^eP values were determined using a one-tailed, paired Student’s t-test.