Figure 7.

Effect of actinomycin and hapten on accumulation of MCP-1 mRNA. The values shown are the net differences between the mRNA for the unstimulated vs. stimulated cells. The data are the pooled results from two experiments in which samples for each time point were collected and from a third experiment in which no samples were collected at 100 and 120 min. The data for the cells to which no antigen was added are shown at time “zero” even though they were collected at 60 min. The values for the MCP-1 mRNA were corrected for the content of β-actin mRNA and then normalized to the amount found at 80 min. (□) Positive controls; no inhibitor added. (Upper) Actinomycin added at 60 min (■). (Lower) Hapten added at 60 min (■).