Table 1.
Comparative responses of cells stimulated with slowly or rapidly dissociating ligands
| DNP
|
NP
|
Relative response*
|
||||
|---|---|---|---|---|---|---|
| High dose | Low dose | High dose | Low dose | High dose | Low dose | |
| PY-FcɛR | 1 | 0.21 | 1.01 | 0.28 | 1.01 | 1.3 |
| PY-Syk | 1 | 0.70 | 0.54 | 0.29 | 0.54 | 0.42 |
| Degranulation | 1 | 0.94 | 0.34 | 0.28 | 0.34 | 0.30 |
| c-fos mRNA | 1 | 0.88 | 0.45 | 0.21 | 0.45 | 0.24 |
| MCP-1 mRNA | 1 | 1.26 | 1.24 | 0.94 | 1.24 | 0.75 |
| MCP-1 secretion | N.D. | 1 | N.D. | 0.95 | — | 0.95 |
Cells were stimulated with either 50 (high) or 10 (low) ng/ml of the DNP antigen or 500 (high) or 100 (low) ng/ml of the NP antigen. The responses were integrated over time and then normalized to the response of the cells exposed to the high dose of the DNP antigen. N.D., no data.
*
Response to NP/response to DNP at high or low dose of antigen.