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. Author manuscript; available in PMC: 2013 Apr 12.
Published in final edited form as: Neuron. 2012 Apr 12;74(1):65–78. doi: 10.1016/j.neuron.2012.01.031

Figure 8. Par-3 is essential for restricting Notch activity to the basal daughter thereby limiting self-renewal.

Figure 8

(A–F)her4.1 (A–B) or dla(D–E) expression in control (A,D) and par-3 (B,E) morphants. (C) and (F) are quantifications. *** p < 0.001 vs apical, t-test. (G–L) Expression of her4.1(G–H), Hu (I–J), and dla(K–L) in control (G,I,K) or par-3 morphants (H,J,L). (M) Quantitative RT-PCR shows relative fold change of her4.1, her6, dla, dld, notch1a and notch1b in control versus par-3 morphants. * p < 0.05, ** p < 0.01, vs Ctrl MO. (N-R) Transplantation similar to that described in Figure 5B was carried out. Representative image of 4-cell clones derived from control (N) or par-3 morphants (O–Q) in otherwise wild-type brains. In control, the single clone is composed of 1 progenitor (red) and 3 neurons (green), whereas par-3 –deficient clones contained fewer neurons (green) and more progenitors (red). (R) Quantification for N–Q. ** p < 0.01, vs Ctrl MO, Z-test. (S) A model for regulated self-renewal and differentiation in asymmetrically dividing radial glia progenitors. See Discussion. See also Figure S7.