Table 1.
Expression of ABC transporters in the immortalised human brain microvascular endothelial cell line hCMEC/D3
| Gene | Assay ID | Expression | Normoxic ΔCt(SD) | RQ (RQ Range) | ||
|---|---|---|---|---|---|---|
| ABCA1 |
Hs00194045_m1 |
● |
16.31 |
(0.58) |
0.65 |
(0.44-0.97) |
| ABCA2 |
Hs00242232_m1 |
●● |
15.38 |
(0.54) |
0.42 |
(0.29-0.62) |
| ABCA3 |
Hs00184543_m1 |
●● |
14.83 |
(0.61) |
7.85 |
(5.15-11.97) |
| ABCA4 |
Hs00184367_m1 |
● |
17.21 |
(0.62) |
2.20 |
(1.43-3.38) |
| ABCA5 |
Hs00363322_m1 |
●●● |
12.27 |
(0.46) |
6.54 |
(4.75-9.01) |
| ABCA6 |
Hs00365329_m1 |
●●● |
12.82 |
(0.49) |
22.92 |
(16.37-32.09) |
| ABCA7 |
Hs00185303_m1 |
●● |
15.77 |
(0.47) |
n.a.a |
|
| ABCA8 |
Hs00200350_m1 |
● |
17.90 |
(0.93) |
0.31 |
(0.16-0.60) |
| ABCA9 |
Hs00329320_m1 |
● |
17.94 |
(0.58) |
0.66 |
(0.44-0.98) |
| ABCA10 |
Hs00365268_m1 |
● |
17.04 |
(0.61) |
1.30 |
(0.85-1.98) |
| ABCA12 |
Hs00292421_m1 |
n.e.b |
|
|
|
|
| ABCA13 |
Hs00541549_m1 |
n.e. |
|
|
|
|
| ABCB1 |
Hs00184491_m1 |
●●● |
11.91 |
(0.40) |
29.84 |
(22.55-39.48) |
| ABCB2 |
Hs00184465_m1 |
●●● |
12.44 |
(0.40) |
9.68 |
(7.31-12.81) |
| ABCB3 |
Hs00241060_m1 |
●●● |
13.16 |
(0.43) |
20.05 |
(14.90-26.98) |
| ABCB4 |
Hs00240956_m1 |
n.e. |
|
|
|
|
| ABCB5 |
Hs00698751_m1 |
n.e. |
|
|
|
|
| ABCB6 |
Hs00180568_m1 |
●● |
14.08 |
(0.43) |
2.62 |
(1.95-3.51) |
| ABCB7 |
Hs00188776_m1 |
●●● |
13.47 |
(0.36) |
6.02 |
(4.70-7.72) |
| ABCB8 |
Hs00185159_m1 |
●● |
15.29 |
(0.46) |
4.45 |
(3.23-6.13) |
| ABCB9 |
Hs00608640_m1 |
●● |
15.71 |
(0.71) |
0.92 |
(0.56-1.50) |
| ABCB10 |
Hs00429240_m1 |
●● |
14.18 |
(0.37) |
3.00 |
(2.33-3.87) |
| ABCB11 |
Hs00184824_m1 |
n.e. |
|
|
|
|
| ABCC1 |
Hs00219905_m1 |
●●● |
12.76 |
(0.47) |
20.95 |
(15.10-29.07) |
| ABCC2 |
Hs00166123_m1 |
● |
18.41 |
(0.43) |
0.44 |
(0.33-0.59) |
| ABCC3 |
Hs00358656_m1 |
●● |
14.44 |
(0.42) |
8.23 |
(6.17-10.98) |
| ABCC4 |
Hs00195260_m1 |
●●● |
12.49 |
(0.38) |
8.64 |
(6.64-11.24) |
| ABCC5 |
Hs00194701_m1 |
● |
17.44 |
(0.51) |
3.06 |
(2.15-4.37) |
| ABCC6 |
Hs00184566_m1 |
n.e. |
|
|
|
|
| ABCC7 |
Hs00357011_m1 |
n.e. |
|
|
|
|
| ABCC8 |
Hs00165861_m1 |
n.e. |
|
|
|
|
| ABCC9 |
Hs00245832_m1 |
n.e. |
|
|
|
|
| ABCC10 |
Hs00375716_m1 |
●● |
15.14 |
(0.45) |
3.72 |
(2.72-5.10) |
| ABCC11 |
Hs00261567_m1 |
n.e. |
|
|
|
|
| ABCD1 |
Hs00163610_m1 |
● |
17.80 |
(0.77) |
11.53 |
(6.77-19.64) |
| ABCD2 |
Hs00193054_m1 |
n.e. |
|
|
|
|
| ABCD3 |
Hs00161065_m1 |
●●● |
13.41 |
(0.36) |
10.20 |
(7.94-13.11) |
| ABCD4 |
Hs00245340_m1 |
●● |
14.87 |
(0.45) |
4.59 |
(3.37-6.25) |
| ABCE1 |
Hs00759267_s1 |
●●● |
11.80 |
(0.34) |
10.10 |
(7.97-12.79) |
| ABCF1 |
Hs00153703_m1 |
●● |
15.21 |
(0.44) |
2.44 |
(1.80-3.31) |
| ABCF2 |
Hs00606493_m1 |
●●● |
11.97 |
(0.47) |
3.22 |
(2.33-4.46) |
| ABCF3 |
Hs00217977_m1 |
●●● |
13.58 |
(0.32) |
4.21 |
(3.36-5.27) |
| ABCG1 |
Hs00245154_m1 |
●● |
15.48 |
(0.36) |
1.05 |
(0.82-1.35) |
| ABCG2 |
Hs00184979_m1 |
●● |
14.84 |
(0.50) |
1.21 |
(0.85-1.72) |
| ABCG4 |
Hs00223446_m1 |
n.e. |
|
|
|
|
| ABCG5 |
Hs00223686_m1 |
n.e. |
|
|
|
|
| ABCG8 | Hs00223690_m1 | n.e. | ||||
a) ABCA7 mRNA could not be detected in human reference RNA calibrator
b) n.e., not expressed