Figure 5. Denaturant-dependencies of normalized amplitudes and average lifetime of the tri-exponential fluorescence decay curves of A488 of dye-labeled apoflavodoxin.

(A) Denaturant-dependence of normalized amplitudes α₁ (red diamonds), α₂ (blue squares) and α₃ (orange dots). Corresponding fluorescence lifetimes are: τ₁ = 0.439 ns, τ₂ = 4.150 ns, and τ₃ = 3.161 ns. (B) Denaturant-dependence of average lifetime <τ>. To calculate <τ>, equation 9 is used.