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. 2012 Oct 8;7(10):e46826. doi: 10.1371/journal.pone.0046826

Figure 3. Calcium chelation by exogenous DNA and alginate induces expression of the T3SS in P. aeruginosa.

Figure 3

P. aeruginosa PAO1 exoS-lux was used as a reporter for the T3SS. Planktonic cultures were supplemented with (A) exogenous DNA or (B) alginate and various concentrations of calcium and magnesium to BM2 with a baseline concentration of 0.2 mM calcium. Gene expression was measured every 20 minutes for 18 hours and the maximal gene expression is shown. The values shown are the means from experiments done in triplicate and the error bars represent the standard deviation. Values that differ significantly (p<0.02 by unpaired t-test) from the controls (BM2 alone) are marked with an asterisk. Each experiment was performed at least three times and representative values are shown.