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. 2012 Oct 8;7(10):e47300. doi: 10.1371/journal.pone.0047300

Figure 7. TFF1 in HT29-MTX-E12 cells interacts with H. pylori LPS.

Figure 7

A H. pylori total cell lysate (Lane 1)and purified LPS (Lane 2) were electrophoresed on SDS PAGE and (A) stained with Coomassie blue which stains proteins or alcian blue which stains carbohydrate moieties or (B and C) transferred onto PVDF membrane and probed with a total cell lysate made from uninfected HT29-MTX-E12 cells. The filter was then probed with either an antibody to TFF1 (B) or an antibody to TFF3 (C) and a horseradish peroxidase conjugated secondary antibody. Strong TFF1 binding and weak TFF3 binding to low molecular weight RF LPS of H. pylori was detected using the ECL system from Amersham. Identical overlay blots (controls) were probed with just the secondary antibody. This confirmed that only the low molecular weight (6 kDa) band was specific for TFF1 and TFF3 binding.