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. 2012 Oct 8;7(10):e47300. doi: 10.1371/journal.pone.0047300

Figure 8. H. pylori mutants producing truncated LPS do not interact with TFF1 and exhibit reduced colonization of HT29-MTX-E12 cells.

Figure 8

(A) LPS from H. pylori strains PA4 and PA4ΔHP1191 was run on SDS PAGE and stained with alcian blue or blotted onto PVDF membrane and probed with either anti Lewis x antibody or with dimeric TFF1. (B) H. pylori strains PA4ΔHP1191 and P12ΔHP1191 exhibited significantly reduced colonization of HT29-MTX-E12 cells compared with WT parental strains (*p<0.05). (C) Incubation of cells with purified LPS from wild type strain PA4 but not with LPS from strain PA4ΔHP1191 reduced subsequent colonization of the cells with strain PA4 (*p<0.05).